Authors:
Y. M. DOBRYNYA, graduate student, North-Caucasian Federal University,
L. D. TIMCHENKO, doctor of veterinary sciences, professor, head of laboratory,
N. I. BONDAREVA, candidate of biology science, senior researcher,
S. I. PISKOV, candidate of biology science, leading researcher,
Problem Research Laboratory “Experimental immunomorphology, immunopathology, immunobiotechnology” in the center of collective use of scientific equipment, North-Caucasian Federal University (1 Pushkina Str., 355009, Stavropol; tel.: +7 918 751-79-96, +7 905 417-30-22, +7 906 442-75-17, +7 905 419-14-82; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.).
Abstract. Nowadays there is no doubt that the general condition of the microflora of the gastrointestinal tract directly affects on the immunity of the macroorganism, one of the diagnostic criteria for its evaluation are the indices of nonspecific immunity, including phagocytosis. The study was devoted to the phagocytic activity of blood neutrophils (phagocytic index, phagocytic number, completion rate of phagocytosis) of rats under the conditions of experimental antibiotic-associated dysbacteriosis with the influence of a biologically active substance from zooglea of the natural symbiont Medusomyces gisevii (tea fungus) prepared according to our technologies. The animals of both groups were induced with dysbacteriosis by administration of gentamycin sulfate orally for 10 days. Rats of group 2 in addition to the main diet were fed with a substance from the zooglea of the tea fungus, and the rats of group 1 were not fed with the substance. It was found that the inhibition of the microflora of the gastrointestinal tract in the rats of group 1 led to a significant decrease in all indices of phagocytic activity, while the use of a biologically active substance from the zooglea of the tea fungus to animals of group 2 made it possible to avoid a significant decrease in the phagocytic activity of neutrophils, compared with those in group 1, with other equal conditions, and even raise the phagocytic index, which indicates its immunomodulatory properties. This fact makes possible to suggest the expediency of using a substance from the zooglea of Medusomyces gisevii, prepared according to our technology, during dysbacteriosis in animals.
Keywords: dysbacteriosis, phagocytic activity, neutrophils, immunity, tea fingus, Medusomyces gisevii, rats.
