Authors: M. V. Petropavlovskiy, N. A. Bezborodova, A. S. Romanova, A. V. Lysov, V. V. Kozhukhovskaya
Ural Federal Agrarian Scientific Research Centre of Ural Branch of the Russian Academy of Science, Ekaterinburg, Russia E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract. Scientific novelty. The article presents the materials of long-term work on the use of PCR diagnostics together with aserological methods (rid, ELISA) in the study of bovine leukemia virus of various physiological groups, as well as experimental animals (rabbits, small cattle).The aim of the study was to evaluate the PCR method in the diagnosis of bovine leukemia virus. Methods. Biomaterials (blood, colostrum) from cattle for PCR studies were obtained from 20 agricultural organizations of Tyumen, Chelyabinsk, Kurgan regions and the Republic of Bashkortostan. A total of 1,269 blood samples and 36 colostrum samples were examined by PCR. Laboratory methods (rid, ELISA, PCR) were used in the early diagnosis of leukemia virus in young cattle, as well as in the diagnosis of adult livestock. Results. The results obtained by PCR revealed carriers of the Lekota virus in calves aged 15 days to 1 month among young animals, which significantly reduced the duration of recreational activities (an average of 20 %). It was established that serological examination of newborn calves for the presence of antibodies to leukemia virus can give false results due to the influence of high concentrations of maternal antibodies and the development of immunological tolerance. PCR studies of biomaterial (samples 1023), taken from adult cattle (cows 3–4, 6 years), showed that 52 % of the samples were present leukemia virus, which was confirmed by serological studies. It is proved that up to 20% of virus carriers from the number of rid-negative animals are detected during ELISA and PCR. Some types of BLV leukemia can not be detected by serological studies, but easily cope with PCR. The article deals with various types of PCR used in the diagnosis of leukemia, describes the data obtained from experiments on artificially infected animals with leukemia virus.
Keywords: leukemia virus, cattle, cows, PCR diagnostics, immunological tolerance, virus transmission, virus types.
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For citation: Petropavlovskiy M. V., Bezborodova N. A., Romanova A. S., Lysov A. V., Kozhukhovskaya V. V. Opyt primeneniya polimeraznoy tsepnoy reaktsii pri diagnostike virusa leykoza krupnogo rogatogo skota i eye effektivnost’ na raznykh etapakh provedeniya ozdorovitel’nykh meropriyatiy [Experience in the use of polymerase chain reaction in the diagnosis of bovine leukemia virus and its effectiveness at different stages of health activities] // Agrarian Bulletin of the Urals. 2019. No.12 (191). Pp. 52‒59. DOI: 10.32417/1997-4868-2019-191-12-52-59. (In Russian.)
