A. А. Korostylev, L. А. Logvinenko, O. М. Shevchuk, A. P. Divakov
Nikitsky Botanical Gardens – National Science Center of the Russian Academy of Sciences, Yalta, Russia
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Volume 26 No. 2
Date of paper submission: 17.12.25, date of review: 30.12.2025, date of acceptance: 16.01.2026.
Published: 02/28/2026
Abstract. The paper presents data on the degree of rooting and some features of the formation of adventitious roots of stem cuttings of two promising tropical medicinal plant species – Aerva lanata and Orthosiphon aristatus. The purpose of the study was to investigate the features of rhizogenesis of stem cuttings depending on the place of harvesting and treatment with the rooting stimulant indole-butyric acid. Methods. The two-factor experiment was conducted in the period 2019–2021 on the basis of the Nikitsky Botanical Garden according to the generally accepted methods of vegetative plant propagation and was analyzed using the method of dispersion analysis. The scientific novelty of the work lies in the comprehensive comparative assessment of species-specific differences in rhizogenesis in two medicinal tropical taxa, taking into account the location of the plant cuttings and the methods of their treatment with stimulants. The results showed pronounced interspecific differences: Aerva lanata is characterized by low rooting capacity (root formation level < 30 %) and is classified as a difficult-to-root species; Orthosiphon aristatus is characterized by high rooting capacity (> 70 %) and is classified as an easy-toroot species. At the same time, for A. lanata, treatment of lower cuts with an aqueous solution of indolylbutyric acid (IBA) at a concentration of 50 mg/l significantly increased rooting and biometry; for O. aristatus, the use of IBA at a concentration of 50 mg/l did not result in a stable and statistically significant improvement in all parameters. Aerva lanata cuttings harvested from the base of the shoots showed better reproductive and biometric performance compared to cuttings from the middle part of the shoots. In Orthosiphon aristatus, the results did not depend on the order of the shoots; there was no significant advantage of first-order cuttings over second-order cuttings. To increase the degree of rooting of A. lanata cuttings and their biometric parameters, it is recommended to use IMC at a concentration of 50 mg/l, especially for cuttings from the base of the shoots. For O. aristatus, both IMC treatment at a concentration of 50 mg/l and dusting with a mixture of activated charcoal and IMC (0.5 g/kg) can be used, although there is no significant difference in the studied parameters compared to water treatment.
Keywords: Aerva lanata Juss., pol-pala, Orthosiphon aristatus (Blume) Miq., kidney tea, medicinal plants, vegetative propagation
Acknowledgements. The study was carried out as part of a government assignment: “Identification of the patterns of synthesis of biologically active substances as the basis for creating varieties of essential oil and medicinal plants – sources of valuable plant raw materials and means for improving the quality of human life as part of the import substitution program” (FNNS-2025-0001).
For citation: Korostylev A. A., Logvinenko L. A., Shevchuk O. M., Divakov A. P. Application of a root formation stimulant and some features of rhizogenesis in stem cuttings of Aerva lanata and Orthosiphon aristatus during vegetative propagation. Agrarian Bulletin of the Urals. 2026; 26 (02): 237‒246. https://doi.org/10.32417/1997-4868-2026-26-02-237-246 (In Russ.)
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