I. A. Kapitova
Federal Horticultural Research Centеr for Breeding, Agrotechnology and Nursery, Moscow, Russia
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Volume 26 No. 1
Date of paper submission: 01.07.2025, date of review: 17.10.2025, date of acceptance: 06.11.2025.
Published: 01/30/2026
Abstract. One of the key stages of clonal micropropagation is the adaptation of microplants to non-sterile conditions. The main objective of this stage is to increase the survival rate of microplants during their adaptation to unfavorable environmental factors. The purpose of the study was to investigate the effect of plant growth regulators on survival and subsequent vegetative development of garden strawberry (Fragaria × ananassa Duch.) cultivars Nashe Podmoskovye and Studencheskaya, as well as the hybrid crop wild strawberry (Kupchikha). Methods. The studies were conducted in accordance with methodological guidelines for clonal micropropagation. Results. The effects of the diphenylurea-type cytokinin forchlorfenuron (CPPU) at concentrations of 0.1 and 0.2 mg/L and the plant growth retardant chlormequat chloride (CCC) at concentrations of 1.0 and 0.2 mg/L were analyzed. A positive effect of chlormequat chloride at a concentration of 1.0 mg/L on the survival of garden strawberry microplants at the stage of adaptation to non-sterile conditions was established, with survival rates 1.5–1.7 times higher than the control. In zemklunika, survival ranged from 83.3 % to 100 %. Improvement of growth parameters of garden strawberry microplants treated with chlormequat chloride at 1.0 mg/L was observed, including an increase in the number of leaves during the growing season by 1.2–1.3 times, petiole length by 1.2 times, and the number of stolons by 1.8 times. For certain parameters (number of leaves and stolon formation), treatments with forchlorfenuron at 0.1 and 0.2 mg/L exceeded the control. In wild strawberry, treatments with forchlorfenuron at 0.1 and 0.2 mg/L and chlormequat chloride at 1.0 mg/L showed positive effects for most biometric parameters. An increase in stolon formation in wild strawberry was noted when forchlorfenuron was applied, with a 1.3–1.4-fold increase compared to the control by the 10th week after transfer to non-sterile conditions. High concentrations of chlormequat chloride (2.0 mg/L) had a negative effect on the development of garden strawberry plants (reduction in leaf number and stolon formation) and wild strawberry (all studied biometric parameters). No such negative effects were observed when plants were treated with forchlorfenuron at 0.1 and 0.2 mg/L or with chlormequat chloride at 1.0 mg/L. Scientific novelty. This study is the first to provide a comparative analysis of the effect of different concentrations of growth regulators on the development of strawberry and wild strawberry microplants during the adaptation stage.
Keywords: clonal micropropagation, garden strawberry, wild strawberry, plant growth regulators, chlorcholine chloride (CCC), forchlorfenuron (CPPU), adaptation
Acknowledgements. The research was carried out within the state order of FSBSO ARHCBAN for the topic “Creation of a methodological platform of biotechnological processes and development of element of technological for management of genetic of selection forms if fruit and berry crops” (FGUW-2025-0001).
For citation: Kapitova I. A. Effect of plant growth regulators on the development of garden strawberry and wild strawberry microplants at the stage of adaptation to non-sterile conditions and during their further growing. Agrarian Bulletin of the Urals. 2026; 26 (01): 118‒128. https://doi.org/10.32417/1997-4868-2026-26-01-118-128 (In Russ.)
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