Authors: E. S. Avramova, O. E. Cherepanova
Botanical Garden of Ural Branch of the Russian Academy of Sciences, Ekaterinburg, Russia, E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract. Hedysarum gmelinii is a perspective species for the introduction into the culture and further use in the agricultural industry and pharmacology. The purpose of this research is to find suitable medium for seed germination. At present, this species is under the threat of extinction in several subjects of the Russian Federation. That is why research of the optimal nutrient medium for the seeds of H. gmeliniiis very relevant. Materials and methods: 100 pcs of mature seeds were selected from the populations of Tatarstan and Bashkortostan. The seed material had been stored at the 4 °C before it was used. The Murashige and Skoog (MS) medium was prepared under the protocol with adding of such plant hormones like 6-benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in four types of concentrations. After that the sterilized seeds were planted. The germination was occurred at 20 °С and the daylight was 16 hours. The results: seeds started to grow up to 15th day of an experiment. Then the plants were transferred on the fresh medium. Young plants were formed after 13 days. Plants which seeds had been taken in Tatarstan showed the most active callus formation. Thus, the originality of this research is that the optimal medium for growing seeds of Hedysarum gmelinii in vitro was found. This medium ensures good viability and gives the opportunity to get the tissues culture for the further studies of the biologically active substances.
Keywords: 6-benzylaminopurine (BAP), Indole-3-acetic acid (IAA), Murashige and Skoog medium, seeds germination, Hedysarum gmelinii Ledeb., Fabaceae, establishment in vitro, callus, pharmacology, agricultural industry, threat under extinction.
For citation: Avramova E. S., Cherepanova O. E. Vvedenie v kul’turu in vitro Hedysarum gmelinii Ledeb. [Establishment of in vitro culture of Hedysarum gmelinii Ledeb.] // Agrarian Bulletin of the Urals. 2020. No. 10 (201). Pp. 35–42. DOI: 10.32417/1997-4868-2020-201-10-35-42. (In Russian.)
