Authors:
V. A. Makutina,
R. A. Vazirov,
A. S. Krivonogova,
I. M. Donnik,
A. G. Isaeva,
M. V. Petropavlovskiy
Ural Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of Sciences, Ekaterinburg, Russia
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Abstract. The purpose of this study was to evaluate the effects of low-dose of ionizing radiation (IR) and nonionizing radiation on oocyte in vitro maturation (IVM) and subsequent embryonic development. Materials and methods. In an experimental study, bovine ovaries of the first group were irradiated with an electron accelerator. The ovaries of the second group were located inside of a coil and were exposed to a magnetic field. After irradiation, oocytes were obtained from the ovaries and IVM and in vitro fertilization (IVF) were performed, followed by observation of embryo development in a time-laps system. The embryos of the third group were exposed to electromagnetic radiation (EMR) from the router (2.4 GHz) throughout the entire period of embryo cultivation from fertilization to blastocyst stage. Results. The obtained results of irradiation of the ovaries did not allow us to reliably state if there is a presence of a negative effect of exposure to small doses of irradiation and electromagnetic radiation. However, in both experimental IR groups, there was a decrease in the level of formed blastocysts compared to the control group. The effect of a magnetic field on the ovaries causes a small but significant increase in the timing of the first embryo cleavage. In addition, there was a trend towards a decrease in the number of mature oocytes and formed blastocysts, indicating an increase in the level of degeneration of bovine oocytes and embryos. The direct exposure of preimplantation embryos to EMR did not influence on embryos development and did not reduce the number of blastocysts formed in vitro. Scientific novelty. We have carried out a comparative analysis of the influence of low-dose ionizing radiation on the development of pre-implantation bovine embryos in vitro. We have studied the influence of electromagnetic radiation from anthropogenic sources on the embryo cleavage.
Keywords: bovine oocyte, in vitro maturation, in vitro fertilization, magnetic field, electromagnetic radiation
For citation: Makutina V. A., Vazirov R. A., Krivonogova A. S., Donnik I. M., Isaeva A. G., Petropavlovskiy M. V. Biological effects of exposure to ionizing and non-ionizing radiation on the preimplantation bovine embryos development. Agrarian Bulletin of the Urals. 2024; 24 (10): 1322‒1333. DOI: https://doi.org/10.32417/1997-4868- 2024-24-10-1322-1333. (In Russ.)
Acknowledgements. The study was supported by a grant from the Russian Science Foundation, project No. 19-76- 10022, https://rscf.ru/project/19-76-10022.
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