S. E. Nizkiy, A. E. Miroshnichenko
Federal Scientific Center “All-Russian Scientific Research Institute of Soybeans”, Blagoveshchensk, Russia
E-mail: Agrofak06 @mail.ru
Volume 25 No. 11
Date of paper submission: 17.02.2025, date of review: 15.05.2025, date of acceptance: 18.08.2025.
Published: 11/30/2025
Abstract. This study investigates the effects of prolonged flooding on the photosynthetic apparatus of soybeans, focusing on the dynamics of chlorophyll a and b content in the leaves of 8 different soybean cultivars. The research is particularly relevant due to the agricultural significance of soybeans and their sensitivity to soil waterlogging, especially in regions with unstable water regimes, such as the Russian Far East. Purpose. The study aimed to assess the adaptive potential of various soybean genotypes to flooding stress by analyzing changes in photosynthetic pigment content. Methods. The experiment was conducted under controlled laboratory conditions using a hydroponic phytosystem. Plants were grown in plastic pots with soil maintained at optimal moisture levels in the control group and in a flooded state in the experimental group. Flooding was initiated at the beginning of the flowering phase and maintained for 15 days, during which leaf samples were collected at various stages of reproductive development. Chlorophyll content was determined spectrophotometrically from alcoholic extracts. The results revealed significant genotypic variability in the soybean response to flooding. A general trend was observed: most cultivars exhibited an increase in chlorophyll a content during short-term flooding (3–6 days), followed by a decrease or stabilization. However, some genotypes maintained stable or even increased chlorophyll a levels throughout the entire stress period. The dynamics of chlorophyll b content were more variable but generally showed an initial increase in the early stages of flooding, followed by a decline, sometimes leading to complete depletion with prolonged exposure. The analysis of chlorophyll dynamics enabled the classification of the studied cultivars into groups based on their resistance to flooding. Scientific novelty. The criterion of soybean resistance to waterlogging by analyzing changes in the content of photosynthetic pigments has been developed for the first time.
Keywords: soybean, flooding, waterlogging, chlorophyll a, chlorophyll b, photosynthetic pigments, stress resistance, soybean breeding
For citation: Nizkiy S. E., Miroshnichenko A. E. Soybean leaf chlorophyll content dynamics as a parameter for assessing flood stress tolerance. Agrarian Bulletin of the Urals. 2025; 25 (11): 1783‒1791. https://doi.org/10.32417/1997-4868-2025-25-11-1783-1791 (In Russ.)
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