N. N. Doguzova, I. A. Gazdanova, A. R. Pukhaev
Federal Scientific Center “Vladikavkaz Scientific Center of the Russian Academy of Sciences”, Vladikavkaz, Russia
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Volume 25 No. 12
Date of paper submission: 25.06.2025, date of review: 30.07.2025, date of acceptance: 29.09.2025.
Published: 12/31/2025
Abstract. Potato late blight caused by the dangerous pathogen Phytophthora infestans is a global problem that annually causes enormous economic damage to agriculture. The fight against this disease requires constant efforts aimed at developing new and improving existing control methods. The aim of the work was to identify resistance to late blight in potato hybrids using molecular markers of Rpi genes. Methods. DNA for molecular genetic analysis was isolated from plant leaves collected during the growing season using the CTAB method. The following DNA markers were used in the molecular analysis of potatoes: R1-1250/R1, R3a-1380/R3a; R3b-378/R3b. They do not provide a complete picture of the genetic diversity of the pathogen population and trace evolutionary changes over time and space. According to the results of the research, a complete set of 3 markers (R1-1250/R1, R3a-1380/R3a and R3b-378/R3b) was found in 3 hybrid potato lines: 3234 (obtained from crossing the potato varieties Palace × Labadia), 3214c (Jewel × Sprinter), and 3190 (Sanibel × Ferrari). This indicates a high degree of resistance of these hybrids to late blight, since the presence of all three genes provides a comprehensive reliable mechanism of protection against the disease. 8 potato genotypes: 3290 (twice presented hybrid Jewel × Krepysh), 3264 (Carmen × Elmundo), 3267 (Triumph × Elmundo), 3234 (Palace × Labadia), 3314 (Riviera × Gala), 3244 (Charoit × Labadia), and 3208 (Carmen × Caprice) showed the presence of markers R1-1250/R1 and R3b-378/ R3b. The study showed that the presence of a combination of markers R1-1250/R1, R3a-1380/R3a and R3b-378/ R3b is the most desirable trait for ensuring high resistance of potato to late blight. Conclusions. Only such a comprehensive approach using molecular markers will allow us to combat late blight more effectively and ensure food security in the future. Further research should be aimed at clarifying the mechanisms of resistance formation, finding new targets, studying the role of genetic flow in the spread of the pathogen, and developing predictive models for assessing the risk of epiphytoties. The scientific novelty of the study lies in the fact that an assessment was made of the resistance of hybrid potato material to Phytophthora infestans.
Keywords: DNA, potato, hybrid, late blight, resistance, Rpi genes, molecular markers
For citation: Doguzova N. N., Gazdanova I. A., Pukhaev A. R. Evaluation of promising potato hybrids for resistance to Phytophthora infestans. Agrarian Bulletin of the Urals. 2025; 25 (12): 2033‒2042. https://doi.org/10.32417/1997-4868-2025-25-12-2033-2042 (In Russ.)
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