Authors:
V. A. Bessonova, O. E. Cherepanova
Botanical Garden of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg, Russia
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Abstract. The purpose of this research was to introduce Ginkgo biloba into culture, to study the composition and properties of its biologically active compounds. Methods. We researched the optimal growth conditions for obtaining a viable tissue culture, such as: concentration of phytohormones and other organic and nonorganic substances in Murashige – Skoog medium and light hours. The effectiveness of the standard method of sodium hypochloride sterilization of young leaves and vegetative buds also was verified. As a result, of conducting the experiment we were able to grow a living callus from leaves of G. biloba. Based on this result we can conclude that these conditions are acceptable for high proliferative activity of the plant. We were studied the effect of phytohormones NAA, at a concentration of 0.5 ml and 6-BAP, at a concentration of 2.5 ml. Also, was selected the ideal planting material for callus production – young leaves that were more sensitive to treatment with hypochloride. This research serves as the foundation for future research not only for our laboratory, but also for other research groups. Scientific novelty. The callus can be used to clone specimens of G. bilobain greenhouses. It will be use to extract and study unique chemical compounds, such as ginkgolides, bilobalides and various terpenes, contained in the extract of plants of this group.
Keywords: Ginkgo biloba, callus Ginkgo biloba, concentration of phytohormones, sterilization of Ginkgo biloba, Egb 761, ginkgolides, plant regeneration, secondary metabolites.
For citation: Bessonova V. A., Cherepanova O. E. Vvedenie v kul’turu in vitro Ginkgo biloba (Linnaeus, 1771) [Introduction to in vitro culture of Ginkgo biloba (Linnaeus, 1771)] // Agrarian Bulletin of the Urals. 2020. No. 12 (203). Pp. 43–49. DOI: 10.32417/1997-4868-2020-203-12-43-49. (In Russian.)
