I. A. Kapitova, K. V. Pavlov
Federal Horticultural Research Center for Breeding, Agrotechnology, and Nursery, Moscow, Russia
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Volume 25 No. 2
Date of paper submission: 06.08.2024, date of review: 22.10.2024, date of acceptance: 30.10.2024.
Published: 02/28/2025
Abstract. The purpose of this review was to present the latest advancements in the extraction and purification of anthocyanins, as well as to discuss the effectiveness and efficiency of these methods. Methods. The analysis was conducted on scientific literature, articles, and databases containing information on methods for extracting anthocyanins from various plant sources and purification techniques. Scientific novelty. In recent decades, it has been widely recognized that the daily consumption of medicinal herbs, fruits, and vegetables provides a wide range of health benefits. This beneficial effect is attributed to the composition of these products, which are rich in bioactive compounds capable of modulating various processes in the human body, regulating glucose levels, and strengthening the immune system. In recent decades, researchers worldwide have been studying the diverse biological activities of anthocyanins. Studies have shown that anthocyanins possess anti-cancer, anti-mutagenic, anti-aging properties, and are active in the prevention and treatment of cardiovascular diseases, while being safe and non-toxic. Currently, anthocyanins are widely used as dietary supplements, in cosmetics, health foods, and the food industry. Research on the methods of anthocyanin extraction and purification from plants has recently gained relevance and demand. This review analyzes the advantages and disadvantages of modern and most common methods of anthocyanin extraction and purification. Results. This article critically reviews six types of anthocyanin extraction (solvent liquid extraction, ultrasonic extraction, microwave extraction, supercritical carbon dioxide extraction, deep eutectic solvent extraction or ionic liquids, and enzyme-assisted extraction), as well as their combinations and four purification methods (column chromatography, high-speed counter-current chromatography, membrane filtration, and preparative high-performance liquid chromatography). The review presents the latest advancements in the extraction and purification of anthocyanins, analyzes, and compares the effects of various extraction and purification methods, and methods for determining the extraction rate and purity of anthocyanins.
Keywords: anthocyanins, extraction methods, purification techniques, antioxidants
For citation: Kapitova I. A., Pavlov K. V. Methods of extracting anthocyanins from plant material and purification techniques (review). Agrarian Bulletin of the Urals. 2025; 25 (02): 245‒253. https://doi.org/10.32417/1997-4868-2025-25-02-245-253 (In Russ.)
Download the full text of the article
