L. N. Skipin1 , E. V. Zakharova1 , S. S. Tarasova1, E. P. Evtushkova2
1 Tyumen Industrial University, Tyumen, Russia
2 State Agrarian University of the Northern Trans-Urals, Tyumen, Russia
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Volume 26 No. 2
Date of paper submission: 22.03.2025, date of review: 13.07.2025, date of acceptance: 10.10.2025.
Published: 02/28/2026
Abstract. The territories of Western Siberia are subject to both natural and anthropogenic salinization of soils. The implementation of the biological stage of reclamation on saline soils using legume crops as phytomeliorants will reduce the use of nitrogen fertilizers, followed by the fixation of biological nitrogen by nodule bacteria. The purpose of the study is to identify the optimal and critical levels of natural and technogenic salinity chemistry that affect the vital activity of nodule bacteria in sweet clover and alfalfa in saline nutrient media. Methods. The studies were conducted in model laboratory experiments, where the chemisms and degree of salinity were formed according to neutral, sulfate-soda, and chloride-sulfate with gypsum. The degree of salinity of the bean agar was created from an unsalinated level to a salt marsh. For each variant, the number of colonies was counted and cultural characteristics were determined. Scientific novelty. For the first time, the parameters of nodule bacteria’s vital activity in saline nutrient media, which are characteristic in terms of chemistry and degree of salinity for the saline soils of Western Siberia, have been determined. The influence of the reclamation agent-coagulant on the activity of rhizobia has been established. Results. Model experiments have shown that nodule bacteria have an acute need for calcium in conditions of natural and man-made salinization with neutral and sulfate-soda chemistry, which reduces the indicators (LD50 and LD99) for rhizobia. The application of gypsum as a reclamation agent on saline soils and drilling sludge allows to eliminate the calcium deficiency in the conditions of salinization, to reduce the salinity and toxicity for legume crops-phytomeliorants, and also to improve the physical properties of these objects, in particular, water permeability, porosity, aeration, structure and other indicators. The specified reclamation technique should be mandatory both for the reclamation of saline soils and for sludge ambar.
For citation: Skipin L. N., Zakharova E. V., Tarasova S. S., Evtushkova E. P. The reaction of nodule bacteria to the salinization conditions of soils and drilling mud. Agrarian Bulletin of the Urals. 2026; 26 (02): 278‒288. https://doi.org/10.32417/1997-4868-2026-26-02-278-288 (In Russ.)
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