М. А. Dolzhikova, А. V. Pikunova, А. А. Pavlenko, О. D. Golyaeva
Russian Research Institute of Fruit Crop Breeding, Zhilina village, Orel region, Russia
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Volume 25 No. 6
Date of paper submission: 27.09.2024, date of review: 09.12.2024, date of acceptance: 11.03.2025.
Published: 06/30/2025
Abstract. The great genetic potential of the red currant adaptability to various soil and climatic conditions allows it to be cultivated in almost all regions of Russia. The purpose of the study is to characterize the polymorphism of microsatellite loci for verifying the origin of ELS 1674-30-30 with spontaneously arisen terminal (apical) fruiting and hybrids of the family 2521, in which splitting by type of fruiting is observed and there are hybrids with both lateral (typical for culture) and terminal type of fruiting. Methods. Polymorphism of microsatellite loci of the red currant family was evaluated in laboratory conditions by PCR and by visualization of products in polyacrylamide gels. Scientific novelty. For the first time, the polymorphism of microsatellite loci is evaluated in the red currant family where hybrids with both terminal and lateral fruiting types are present. Results. According to the research results, using the analysis of microsatellite loci, the origin of ELS 1674-30-30 with terminal fruiting from the Niva and Veksne cultivars was confirmed; namely, no alleles were found that were amplified in the selected seedling, but absent in the parent forms, and vice versa. Also, according to the polymorphism of microsatellite loci, the origin of hybrids of the family 2521 was clarified – some of which originated from self-pollination of the maternal form and some from pollination with pollen of a different genotype. Presumably, the origin of the maternal form 1674-30-30 with terminal fruiting type was influenced by mutational variability. Keywords: red currant, DNA, microsatellite markers, terminal (apical) fruiting For citing: Dolzhikova М. А., Pikunova А. V., Pavlenko А. А., Golyaeva О. D. Polymorphism of microsatellite loci in the red currant family with cleavage by lateral and terminal fruiting types. Agrarian Bulletin of the Urals. 2025; 25 (06): 864‒872. https://doi.org/10.32417/1997-4868-2025-25-06-864-872 (In Russ.)
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