Authors:
N. I. ISKANDAROVA, candidate of veterinary sciences, associate professor,
O. G. PETROVA, doctor of veterinary sciences, professor,
E. S. ODEGOV, veterinary doctor,
Ural State Agrarian University (42 K. Libknehta str., 620075, Ekaterinburg).
Abstract. Paramyxoviruses – known pathogens of the CNS and respiratory systems of many host species. In the last few decades, there are quite a lot of new paramyxoviruses that cause devastating diseases in various aquatic and terrestrial animals, including humans. Parainfluenza virus type 3 (PIV3) is an elliptic, single-stranded, negative-polar RNA virus of the genus Respiralirus, of the family Paramyxoviridae. Respiralirus genus includes the viruses of human parainfluenza type 1 and 3 (respectively, HPIV1 and HPIV3), Sendai virus (murine PIV1) and parainfluenza virus-3 (BPIV3). In some cases, when the animals are subjected to high stress loads, infection with BPIV3 can contribute to tissue damage and immunosuppression that leads to severe bronchopneumonia from secondary bacterial infections. Parainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions. Examination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).This is the first characterization of BPIV3 in water buffalo. According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C were found in cattle.
Keywords: parainfluenza-3 cattle, acute respiratory viral infections, buffalo, phylogenetic analysis, epidemiology, sequencing, isolates, genotype.
