Authors:
A. A. ISMAYILOV, post-graduate student,
L. D. TIMCHENKO, doctor of veterinary sciences, professor, chief of laboratory,
N. I. BONDAREVA, candidate of biological sciences, senior researcher,
North-Caucasian Federal University,
Problem Research Laboratory “Experimental immunomorphology, immunopathology, immunobiotechnology” in the center of collective use of scientific equipment, North-Caucasian Federal University (1 Pushkina Str., 355029, Stavropol; tel.: +7 928 024-55-99, +7 905 417-30-22, +7 906 442-75-17; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.).
Abstract. Ozone, due to its powerful disinfecting effect, is increasingly used in various applications. Much attention is paid to the study of the effect of ozone on various types of microorganisms, but the question of its effect on the adhesive potential of bacteria remains poorly known. Meanwhile, the adhesion of eubiotic microorganisms on the epithelial cells of mucosal surfaces is a fundamental factor of colonization resistance, providing stability and protective properties of the microorganisms of the macroorganism. The aim of the article is to consider the effect of ozone on the adhesive properties of lactobacilli. For this purpose, the lactobacillus strain Lactobacillusplantarum 8P-A3 was ozonized at various regimes. The modes of ozonation were determined by the bubbling time, while maintaining constant speed, power and temperature. The concentration of ozone was measured by iodometric titration and when bubbling at a constant rate of 0.5 l/min, 100 % power and 20 °C temperature for 5 minutes was 0.38 mg/l, 2 min — 0.29 mg/l. Adhesive properties were studied on human erythrocytes by the method of V. I. Brilis. The number of adherent microorganisms was counted in a smear stained by Romanovsky-Giemsa. The following adhesion values were evaluated: adhesion coefficient (KA, %), average adhesion index (SPA), microorganism adhesion index (IAM). When ozonizing for two minutes, the average adhesion index decreases by 41.4 %, with five minutes — a decrease of 67.2 %. Based on the analysis of the conducted studies, a dose-dependent effect of ozone exposure on the adhesive properties of Lactobacillusplantarum 8P-A3 was established. With increasing ozone dose within the limits of used concentrations, the adhesiveness of lactobacilli decreases.
Keywords: lactobacillus, adhesion, ozone, adhesive properties, microorganisms.
