Authors:
O. I. LAZAREVA, graduate student,
T. N. SIVKOVA, doctor of biological sciences, associate professor,
T. S. PROKHOROVA, candidate of biological sciences, associate professor,
V. K. BEREZHKO, doctor of biological sciences, professor,
L. A. NAPISANOVA, candidate of biological sciences,
Perm State Agrarian-Technical University named after academician D. N. Pryanishnikov (23 Petropavlovskaya Str., 614099, Perm; tel.: +7 342 240-56-53),
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin (28 B. Cheremushkinskaya Str., 117218, Moscow; tel.: +7 499 124-56-55).
Abstract. In the article the influence of the somatic extract of Anisakis simplex L3 to the cultures of microorganism cells in vitro is considered. The somatic extract from A. simplex L3 disrupts and depresses the process of dividing eukaryotic cells was established earlier. There are very few data on the mechanisms of interactions of somatic extracts of helminths and microorganisms. Somatic extract anisakids renders a negative effect on microorganisms due to its constituent protein components and metabolites, it is supposed. Somatic extract from anisakids has a negative effect on microorganisms due to proteins and metabolites in its composition, it is assumed. The aim of the study was to study the effect extract on cell cultures of microorganisms diverse in morphology and resistance to environmental factors. The extract was prepared from the larvae of anisakids, the larvae were removed from frozen Micromesistius poutassou, then the extract was checked for sterility and harmlessness, the protein content was determined, then the disks were soaked with somatic extract. For the study, daily cultures bacteria were used: micrococci Micrococcus sp., of sticks Escherichia coli, Proteus vulgaris, Salmonella tiphimurium, and bacilli Bacillus subtilis. Microorganisms were cultured with discs that contained an antigenic extract of anisakids in a thermostat at + 37 °C, after 12 hours, a growth retardation zone was detected in Micrococcus sp., E. coli and P. vulgaris. The extract had no effect on the growth of S. tiphimurium, B. subtilis. The sterility zone, which is formed temporarily, indicates that the extract contains biological active components acting bacteriostatically on microorganisms. The mechanisms of bacteriostatic action of the somatic extract of Anisakis simplex L3 on bacteria were by assumed. The obtained results can be used to develop a model of bacterial cells as a test of objects for evaluating the antigenic activity of somatic, excretorysecretory extracts and metabolites of various helminths.
Keywords: somatic extract, Anisakis simplex L3, metabolites, bacteria, bacilli, bacteriostatic action.
