Authors:
O. I. ZAKHAROVA, external doctoral candidate,
E. S. SLEPTSOV, doctor of veterinary science, professor,
N. V. VINOKUROV, candidate of veterinary science, senior researcher,
Yakut Scientific Research Institute of Agriculture named after M. G. Safronov (23/1 B-Marlinskogo Str., 677001, Yakutsk)
Abstract. In the article results of approbation of ELISA based on monoclonal antibodies in the diagnosis of brucellosis of reindeer conducted in the laboratory of reindeer breeding and traditional branches of the Yakut Scientific Research Institute of Agri- culture and on the experimental base of the Russian Scientific Research Institute of Experimental Veterinary of Y. R. Kova- lenko. Positive and questionable reactions with the help of agglutination reaction (AR) + complement fixation reaction (CFR) complex, 29 samples were detected – 8.2 % from the number of investigated, with the help of ELISA-35 samples – 10 % (the ELISA test fully coincided with the readings of AR + CFR, in addition to AR + CFR, 6 samples – 1.7 % of those studied). In all 35 samples with positive and questionable reactions in AR + CFR and/or ELISA, a negative result was obtained in the immunodiffusion reaction (IR) with O-polysaccharide antigen, which indicates the absence of epizootically dangerous ani- mals in the surveyed herds. In ELISA, of the 25 test sera, a positive result of 8 samples was shown (optical density of 1.362 – 2.063), which indicates the complete compliance of the ELISA indicators with the indirect hemagglutination reaction (IHGR) and the diagnostic complex AR + CFR and the greater diagnostic sensitivity of ELISA compared to the indicated antibody detection to brucella.
Keywords: brucellosis, infection process, immunity, strain, epizootic process, vaccine, reindeer.
