Authors:
N. A. ZABOKRITSKIY, doctor of medical sciences, senior researcher, associate professor
INSTITUTE OF IMMUNOLOGY AND PHYSIOLOGY OF THE URAL BRANCH OF THE RUSSIAN ACADEMY OF SCIENCES (106 Pervomayskaya Str., 620049, Ekaterinburg)
D. Y. SAVINYKH, general director,
SCIENTIFIC INDUSTRY CENTER “URALBIOSINTEZ” (2, lit. D Novinskaya Str., 620024, Ekaterinburg)
Abstract. The theoretical researches executed by us, and also long-term experience of conducting pilot studies of the pro-biotic microorganisms, and practical use of the medicines and drugs framed on their basis allowed us to assume a possibility of development of new biologically active substance as a base of new perspective drug for a veterinary medicine. For the solution of an objective it was necessary to conduct first of all researches on the choice of a pro-biotic microorganism, in pilot studies to study its biological properties and based on the received results to make the decision about the expediency of creation of an experimental sample of metabolic substance on its basis. The largest biological potential and a set of positive properties which can effectively be used for development of pro-biotic drugs belong to the non-pathogenic sporiferous microorganisms of the sort Bacillus and in particular various strains of a hay rod. It is established that they are characterized by the expressed opposing properties concerning many types of opportunistic and pathogenic microorganisms, ability to have immunomodulatory effect (generally due to stimulation of various links of cellular and humoral immunity), and also have antitoxic, antiallergenic, antiradiation and other effects. The experimental sample of biologically active substance is developed on the basis of metabolites of specially chosen pro-biotic strain of Bacillus subtilis of V-9906 which is characterized by the high level of production of such biologically active agents as albuminous polysaccharide complex, free amino acids, the purine and pyrimidine bases, B2 and B6 vitamins, proteolytic enzymes, antibiotic-like bonds. At experimental modeling of an acute generalized salmonellosis, the essential augmentation of survival of laboratory animals for whose treatment an experimental sample was used has been discovered. Opposing and biochemical activity, protective and immunotropic properties of the developed metabolic substance are studied.
Keywords: biologically active substance, biochemical and antagonistic activity, protective and immune stimulating properties, the prospect of creating a new drug for veterinary use.
