Authors:
N. A. ZABOKRITSKIY, doctor of medical sciences, senior researcher, associate professor,
INSTITUTE OF IMMUNOLOGY AND PHYSIOLOGY OF THE URAL BRANCH OF THE RUSSIAN ACADEMY OF SCIENCES (106 Pervomayskaya Str., 620049, Ekaterinburg)
O. E. CHEREPANOVA, candidate of biological sciences, researcher,
BOTANIC GARDEN OF THE URAL BRANCH OF THE RUSSIAN ACADEMY OF SCIENCES (202а 8 Marta Str., 620144, Ekaterinburg)
N. N. DUDUKINA, senior researcher,
URAL SCIENTIFIC RESEARCH VETERINARY INSTITUTE (112а Belinskogo Str., 620142, Ekaterinburg)
Abstract. To date, the scientific literature is described in detail a list of substances extracted from various parts of the plant Calluna vulgaris, showing a medicinal properties and being interesting from the medical point of view. The study of medicinal properties the heather which is growing on the territory of our country has begun comparatively recently and was limited to only the territory of the Russian Plain, which occupies large areas of heather. Genetic heterogeneity of the eastern part of its range the heather makes it necessary for studying the qualitative and quantitative composition of the extracts obtained from different populations in order to identify the most valuable populations on their pharmacological properties. There are the results of biologically active substance accumulation seasonal dynamics study (for the quercetin) in the leaves of C. vulgaris, which grows in the subzone of Western Siberia predlesostepare presented in the article. The determination of the seasonal dynamics of accumulation of biologically active substances was performed by reversed-phase HPLC with photometric detection. The evaluation of the biologically active substances accumulation dynamic for the growing season (quercetin accumulation) is revealed that the quercetin content in the population which are grown in the West Siberia more than 2.5 times higher as compared to the populations of the Russian plain. August - September is the best time of preparation of raw materials, when quercetin content as much as possible. Most likely that the increase in quercetin content associated with the number of chloroplasts in the leaf cells, the number of which in turn, also increases in this period. The increase in the linear dimensions of the heather sheets, perhaps due to a decrease of illumination under the forest canopy.
Keywords: Calluna vulgaris, quercetin, leaf, growing season, high performance liquid chromatography.
