A. P. Poryvaeva, A. S. Krasnoperov, O. Yu. Oparina, Y. Yu. Lysova, O. G. Tomskikh, E. A. Loginov, A. G. Isaeva
Ural Federal Agrarian Research Center of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg, Russia
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Volume 25 No. 8
Date of paper submission: 14.05.2025, date of review: 23.06.2025, date of acceptance: 01.07.2025.
Published: 08/31/2025
Abstract. The search for alternative methods of treatment of infectious diseases is still an extremely important direction in the fight against antimicrobial resistance of pathogenic and opportunistic microorganisms. The purpose of study was to investigate the effect of experimental composition of bacteriophages on the course and character of intestinal diseases with symptom complex of diarrhoea in calves aged up to 30 days. Methods. We studied the effect of “Antibacterial composition based on bacteriophage strains” on the course and character of gastrointestinal diseases with symptom complex of dyspepsia in Holstein calves aged up to 30 days. Scientific novelty of study lies in the positive changes in the intestinal microbiome of calves. Results. It was found that application of “Antibacterial composition based on bacteriophage strains” suppresses the growth of pathogenic microflora in the intestine of sick calves – bacteria Staphylococcus spp., Staphylococcus aureus, Proteus spp., Pseudomonas aeruginosa, including their antibiotic-resistant strains. During 14 days the concentration of Staphylococcus spp. decreased by 0.34 ± 0.09 lg; Proteus spp. – by 0.62 ± 0.07 lg; Pseudomonas aeruginosa – by 1.3 ± 0.6 lg. Positive dynamics of Staphylococcus spp. bacteria concentration reduction in calf intestinal microflora was registered up to 35 days of scientific and production experiment inclusive. Bacteria Proteus spp. and Pseudomonas aeruginosa were not detected in the intestinal microflora of calves on the 35th day. The indirect positive effect of “Antibacterial composition based on bacteriophage strains” on calf intestinal microflora – restoration of the number of strict anaerobes and microaerophiles of Lactobacillus, Bifidobacterium, Bacteroides genus (normal flora) was registered. The use of “Antibacterial composition based on bacteriophage strains” reduced the risk of recurrence of gastrointestinal tract diseases with symptom complex of diarrhoea in calves within 2–3 weeks after treatment measures, and in the scheme of treatment of calves with severe and extremely severe degree of gastrointestinal diseases allowed to reduce the intensity of manifestations of symptom complex of dyspepsia and avoid unfavourable outcome of the disease – mortality of young animals.
Keywords: bacteriophage composition, symptom complex of diarrhoea, calves, clinical condition, gut microbiota, immunohaematological studies, serological screening, molecular genetic studies
Acknowledgements. The publication was prepared within the framework of implementation of state assignment in accordance with the Program of Fundamental Scientific research of State Academies of Sciences No. 0532-2021- 0004 and No. 0532-2021-0007.
For citation: Poryvaeva A. P., Krasnoperov A. S., Oparina O. Yu., Lysova Y. Yu., Tomskikh O. G., Loginov E. A., Isaeva A. G. Application of experimental composition of bacteriophages in etiotropic therapy of gastrointestinal diseases with symptom complex of diarrhoea in calves (results of scientific and production experiment). Agrarian Bulletin of the Urals. 2025; 25 (08): 1235‒1257. https://doi.org/10.32417/1997-4868-2025-25-08-1235-1257 (In Russ.)
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