A. Yu. Egovtseva, I. A. Kameneva, A. I. Yakubovskaya, S. F. Abdurashitov, M. V. Gritchin, I. I. Smirnova
Research Institute of Agriculture of Crimea, Simferopol, Russia E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Volume 25 No. 9
Date of paper submission: 24.01.2025, date of review: 31.03.2025, date of acceptance: 03.06.2025.
Published: 09/30/2025
Abstract. The practice of using green manure is gaining popularity within the framework of integrated agricultural systems focused on ecology. The use of microbial preparations based on cellulose-decomposing microorganisms, as well as studies of the quantitative and qualitative composition of saprophytic microbiota involved in the transformation of plant mass in the soil, is relevant. The purpose. The study was aimed at investigating the taxonomic diversity of the southern chernozem microbiome using phacelia as a green manure and cellulolytic association (CA). Methods. The studies were conducted in 2018 and 2019 under field experimental conditions on low-humus southern chernozem on loess-like clays. Phacelia plants in the flowering phase were treated with an aqueous suspension of CA (1 l per hectare) with simultaneous crushing of the phytomass and incorporation into the soil to a depth of 10‒15 cm. Taxonomic analysis of soil samples was carried out by high-throughput sequencing of 16S rRNA gene libraries. The preparation of libraries and sequencing of isolated DNA were carried out on the Illumina MiSeq platform at the Center for Collective Use of Scientific Equipment “Genomic Technologies, Proteomics and Cell Biology” of the All-Russian Research Institute of Agricultural Microbiology (St. Petersburg). Results. Principal component analysis showed that organic substrate and the introduction of a cellulolytic association significantly affect changes in the microbial community at the phyla and genera level. The results of the study indicate the ability of such a technological method as the introduction of phacelia green manure and the use of CA to accelerate decomposition, to influence the change in the taxonomic structure of the prokaryotic community of southern chernozem in steppe conditions. Scientific novelty. For the first time, a metagenomic analysis of the taxonomic structure of the community of soil microorganisms initiated by green manure crops and CA in the conditions of the chernozem of the southern Steppe of Crimea was carried out.
Keywords: microbiome, taxonomic structure, cellulolytic association, green manure, phacelia
Acknowledgements. The study was carried out within the framework of the state assignment of the Ministry of Education and Science of the Russian Federation No. FNZW-2022-0004 “Patterns of functioning of agrophytocenoses of southern chernozem under the influence of various farming systems and biologization techniques aimed at improving soil fertility.”
For citation: Egovtseva A. Yu., Kameneva I. A., Yakubovskaya A. I., Abdurashitov S. F., Gritchin M. V., Smirnova I. I. Taxonomic diversity of the microbiome of southern chernozem under the influence of phacelia green manure and cellulolytic association. Agrarian Bulletin of the Urals. 2025; 25 (09): 1316‒1328. https://doi.org/10.32417/1997-4868-2025-25-09-1316-1328 (In Russ.)
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