Authors:
O. I. ZAKHAROVA, competitor,
E. S. SLEPTSOV, doctor of veterinary sciences, professor,
N. V. VINOKUROV, candidate of veterinary sciences, senior researcher,
Yakut scientific research institute of agriculture named after M. G. Safronov (23/1 B-Marlinskogo Str., 677001, Yakutsk)
Abstract. The article presents materials on the study of antigenic, virulent and immunogenic properties of “deer” cultures in the body of laboratory animals. On dense nutrient media after brooding of lyophilized Brucella cultures, resections were made from strains B. suis 61, B. suis 245, B. suis (deer), B. abortus 19 and B. abortus 54. The most rapid growth was noted in Brucella from strains B. abortus 54 and B. abortus 19, then reduced: B. suis 61, B. suis 245, B. suis (deer). On nutrient media, the most intensive growth was observed in test tubes with Brucella cultures B. abortus 19, B. abortus 54, and B. suis 61. On dense nutrient media after 3 days, brucella grew in the form of small (1–2 mm in diameter), convex, round, correctly designed colonies. In transmitted light, they were translucent, had a smooth moist or oily surface, sometimes with a delicate graininess. In reflected light, the colonies had a greenish-blue hue. The young Brucella culture grew on the trail of a bacteriological loop in the form of a gentle, transparent, glossy, oily plaque with a bluish tinge, especially visible in reflected light when sowing on the surface of mown agar. By the nature of growth, it can be assumed that the cultures from the “deer” strain are less pronounced in comparison with other cultures.
Keywords: Brucellosis, infection process, immunity, strain, epizootic process, vaccine, reindeer.
